目的 研究西瓜藤石油醚提取物对佐剂性关节炎(AA) 小鼠的治疗作用并探讨其机制。方法 采用AA小鼠模型观察西瓜藤石油醚提取物的抗炎作用,按 5 级评分法计算关节炎指数,测量胸腺指数及脾脏指数,测定模型小鼠血清中类风湿因子(RF), 前列腺素E2(PGE2), 环氧化酶-1(COX-1)及环氧化酶-1 (COX-2)的含量和肿瘤坏死因子-α(TNF-α), 白介素-1β(IL-1β)水平。结果 西瓜藤石油醚提取物可明显减轻AA小鼠的足肿胀度,降低关节评分指数(AI),升高胸腺指数,降低脾脏指数。高剂量组能显著降低PGE2, IL-1β, COX-2和COX-1的含量,各剂量组均能显著降低小鼠血清TNF-α和RF水平。 结论 西瓜藤石油醚提取物对AA小鼠具有一定的治疗作用,其机制可能与影响炎症介质的产生和抗氧化作用有关。
Abstract
OBJECTIVE To study the pharmacological function of the petroleum ether extract from Citrullus lanatus vine (PEECLV) in treating adjuvant arthritis(AA) and explore the mechanism. METHODS AA in mice was made to observe the anti-inflammatory effect of PEECLV. Arthritis index was calculated by 5 grades evaluation. Thymus and spleen index were measured. The content of RF, PGE2, COX-1, COX-2 in serum were measured. The levels of TNF-α, IL-1β in serum were measured. RESULTS PEECLV can suppress the foot swelling degree in model mice. It can increase thymus index, reduce spleen index and AI of AA in mice. High-dose group can significantly reduce serum PGE2, IL-1β, COX-2 and COX-1 levels in AA in mice. All groups can reduce TNF-α and RF levels. CONCLUSION PEECLV has a certain degree therapeutic effect on AA, and the possible mechanism is to affect the production of inflammatory mediators and antioxidant.
关键词
西瓜藤 /
石油醚提取物 /
佐剂性关节炎 /
机制
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Key words
Citrullus lanatus vine /
petroleum ether extract /
adjuvant arthritis /
mechanism
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中图分类号：
R965
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参考文献
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脚注
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基金
广西科学研究与技术开发计划项目资助课题(桂科合14123001-2);广西中药药效研究重点实验室开放项目(13-051-06-K4)
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